Association of CCND1 Gene Polymorphism rs9344 with Grade and Invasion Degree of Colorectal Cancer at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital



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Submitted Mar 25, 2023
Published Apr 26, 2023
10.24018/ejbiomed.2023.2.2.58

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The majority of colorectal cancer (CRC) are sporadic CRC that can be caused by genetic variations such as Single Nucleotide Polymorphisms (SNPs). The CCND1 gene polymorphism rs9344 could involve at the beginning and the development of CRC.

This study aimed to analyze the association between CCND1 gene polymorphism rs9344 with the grade and invasion degree of colorectal cancer in at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital, Denpasar, Bali. This cross-sectional study was carried out at the Integrated Biomedical Laboratory Unit, Faculty of Medicine, Udayana University.

Data analysis of 32 samples showed majority age was above 50 years old as many as 28 (87.5%), with men as 21 samples (65.6%). Histopathology description was adenocarcinoma in 32 samples (100%). Grading histopathology low grade was 31 samples (96.6%). The degree of tumor invasion was high as 25 samples (78.1%). From the aspect of pathological stage pNx as much as 27 (84.4%). The majority location of tumors was on the left side as 21 samples (65.6%). The polymorphisms of CCND1 rs9344 genotype sequentially AA as 20 samples (62.5%), AG as 9 samples (28.1%), while GG as 3 samples (9.4%). The statistical analysis found that CCND1 gene polymorphism rs9344 was not associated with colorectal cancer grade (p>0.05), and not associated with invasion degrees of colorectal cancer (p>0.05).

Taken together, we conclude that no significant association between the CCND1 gene polymorphism rs9344 with grade and invasion degree of colorectal cancer at Prof. Dr. I.G.N.G. Ngoerah Central General Hospital, Denpasar, Bali.

 

Keywords: colorectal cancer CCND1 gene cyclin D1 polymorphism

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References

  1. WHO. Classification of tumours 5th edition digestive system tumours [Internet]. 2019. [cited 2022 Oct 22]. Available from: https://dokumen.pub/who-classification-of-tumours-5th-edition-digestive-system-tumours-1-5nbsped-9789283244998.html#WHO+Classification+of+Tumours+Editorial+Board.
     Google Scholar
  2. Ni Nyoman AD, Suksmarini NMPW, Pranata AANS, Rompis AY, Sumadi IWJ. The prevalence of KRAS and BRAF mutation in colorectal cancer patients in Bali. Indones J Biotechnol [Internet]. 2022 Mar; 29;27(1):29. Available from: https://jurnal.ugm.ac.id/ijbiotech/article/view/67506.
    DOI  |   Google Scholar
  3. Xie M, Zhao F, Zou X, Jin S, Xiong S. The association between CCND1 G870A polymorphisms and colorectal cancer risk. Medicine (Baltimore) [Internet]. 2017; Oct;96(42): e8269. Available from: https://journals.lww.com/00005792-201710200-00058.
    DOI  |   Google Scholar
  4. Chauhan P, Kaur P sahib, Babu AM. CYCLIN D1: As a Risk of Breast Cancer. CGC Int J Contemp Technol Res [Internet]. 2020 Dec; 26;3(1):134–8. Available from: https://cgcijctr.com/document/C0301105-FINAL.pdf.
    DOI  |   Google Scholar
  5. Wen J, Xu Q, Yuan Y. Single nucleotide Polymorphisms and sporadic colorectal cancer susceptibility: A field synopsis and meta-analysis. Cancer Cell Int. 2018;18(1).
    DOI  |   Google Scholar
  6. Qiu H, Wang Y, Kang M, Tang W, Chen Y. OncoTargets and Therapy Dovepress investigation of cyclin D1 rs9344 g.a polymorphisms in colorectal cancer: a meta-analysis involving 13,642 subjects. 2016; Available from: http://dx.doi.org/10.2147/OTT.S116258.
    DOI  |   Google Scholar
  7. Xu X, Ni X, Yang G, Luo Z, Niu Y, Shen M. CCND1 G870A polymorphisms and colorectal cancer risk: An updated meta analysis. Mol Clin Oncol [Internet]. 2016 Jun 1 [cited 2023 Feb 5];4(6):1078–84. Available from: http://www.spandidos-publications.com/10.3892/mco.2016.844/abstract.
    DOI  |   Google Scholar
  8. Li Y, Wei J, Xu C, Zhao Z, You T. Prognostic significance of cyclin d1 expression in colorectal cancer: a meta-analysis of observational studies. PLoS One [Internet]. 2014 Apr 11 [cited 2023 Mar 9];9(4). Available from: /pmc/articles/PMC3984178/.
    DOI  |   Google Scholar
  9. Albasri AM, Elkablawy MA, Ansari IA, Alhujaily AS. Prognostic Significance of Cyclin D1 Over-expression in Colorectal Cancer: An Experience from Madinah, Saudi Arabia. Asian Pacific J Cancer Prev. [Internet]. 2019 Aug 1 [cited 2022 Sep 12];20(8):2471–6. Available from: http://journal.waocp.org/article_88705.html.
    DOI  |   Google Scholar
  10. Al-Sohaily S, Biankin A, Leong R, Kohonen-Corish M, Warusavitarne J. Molecular pathways in colorectal cancer. 2012 [cited 2022 Oct 20]; Available from: https://onlinelibrary.wiley.com/doi/10.1111/j.1440-1746.2012.07200.x.
    DOI  |   Google Scholar
  11. Rompis AY, Nyoman N, Dewi A. Aspek Genetik Kanker Kolorektal. J Sains dan Kesehat [Internet]. 2020 Jun 30 [cited 2022 Oct 20];2(3):236–45. Available from: https://jsk.farmasi.unmul.ac.id/index.php/jsk/article/view/145.
     Google Scholar
  12. Qie S, Diehl JA. Cyclin D1, cancer progression, and opportunities in cancer treatment. J Mol Med. 2016 Dec 1;94(12):1313–26.
    DOI  |   Google Scholar
  13. Tetsu O, McCormick F. β-Catenin regulates expression of cyclin D1 in colon carcinoma cells. Nat 1999 3986726 [Internet]. 1999 Apr 1 [cited 2022 Oct 20];398(6726):422–6. Available from: https://www.nature.com/articles/18884.
    DOI  |   Google Scholar
  14. Montalto FI, De Amicis F. Cyclin D1 in Cancer: a molecular connection for cell cycle control, adhesion and invasion in tumor and stroma. cells [Internet]. 2020 Dec 9 [cited 2022 Aug 20];9(12). Available from: https://pubmed.ncbi.nlm.nih.gov/33317149/.
    DOI  |   Google Scholar
  15. Fu M, Wang C, Li Z, Sakamaki T, Pestell RG. Minireview: cyclin d1: normal and abnormal functions. Endocrinology [Internet]. 2004 Dec 1 [cited 2022 Aug 28];145(12):5439–47. Available from: https://academic.oup.com/endo/article/145/12/5439/2499715.
    DOI  |   Google Scholar
  16. Salimi S, Shahrakipour M, Hajizadeh A, Mokhtari M, Mousavi M, Teimoori B, et al. Cyclin D1 G870A polymorphisms: Association with uterine leiomyoma risk and in silico analysis. Biomed Reports. 2017;6(2).
    DOI  |   Google Scholar
  17. Jeon S, Kim Y, Jeong Y, Bae J, Jung C. CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer. Cancers (Basel) [Internet]. 2018 Nov 13;10(11):437. Available from: http://www.mdpi.com/2072-6694/10/11/437.
    DOI  |   Google Scholar
  18. Kowalczyk MM, Barańska M, Fendler W, Borkowska EM, Kobos J, Borowiec M, et al. G870A Polymorphic Variants of CCND1 Gene and Cyclin D1 Protein Expression as Prognostic Markers in Laryngeal Lesions. Diagnostics [Internet]. 2022 May 1 [cited 2022 Aug 7];12(5):1059. Available from: https://www.mdpi.com/2075-4418/12/5/1059/htm.
    DOI  |   Google Scholar
  19. Pabalan N, Bapat B, Sung L, Jarjanazi H, Francisco-Pabalan O, Ozcelik H. Cyclin D1 Pro241Pro (CCND1-G870A) polymorphisms is associated with increased cancer risk in human populations: A meta-analysis. Cancer Epidemiol Biomarkers Prev [Internet]. 2008 Oct [cited 2023 Mar 3];17(10):2773–81. Available : from: https://www.researchgate.net/publication/23307407_Cyclin_D1_Pro241Pro_CCND1-G870A_Polymorphisms_Is_Associated_with_Increased_Cancer_Risk_in_Human_Populations_A_Meta-Analysis.
    DOI  |   Google Scholar
  20. Rahimirad S, Mosallaei M, Salehi R, Shariatpanahi S, Salehi AR. The association between cyclin D1 (CCND1) rs9344 AA genotype and increased risk of colorectal cancer in an Iranian population. Middle East J Cancer. 2020;11(3).
     Google Scholar
  21. Tchakarska G, Sola B. The double dealing of cyclin D1. Cell Cycle [Internet]. 2020 [cited 2023 Mar 12];1–16. Available from: https://hal-normandie-univ.archives-ouvertes.fr/hal-02426294.
     Google Scholar
  22. Sameer AS, Parray FQ, Dar MA, Nissar S, Banday MZ, Rasool S, et al. Cyclin D1 G870A polymorphisms and risk of colorectal cancer: A case control study. Mol Med Rep [Internet]. 2013 Mar 1 [cited 2023 Mar 14];7(3):811–5. Available from: http://www.spandidos-publications.com/10.3892/mmr.2013.1287/abstract.
    DOI  |   Google Scholar
  23. Huang C-Y, Tsai C-W, Hsu C-M, Chang W-S, Shui H-A, Bau D-T. The significant association of CCND1 genotypes with colorectal cancer in Taiwan. Tumor Biol [Internet]. 2015 Aug 26;36(8):6533–40. Available from: http://link.springer.com/10.1007/s13277-015-3347-9.
    DOI  |   Google Scholar
  24. Binabaj MM, Bahrami A, Khazaei M, Avan A, Ferns GA, Soleimanpour S, et al. The Prognostic Value of Small Noncoding microRNA-21 Expression in the Survival of Cancer Patients: A Meta-Analysis. Crit Rev Eukaryot Gene Expr [Internet]. 2020 [cited 2023 Mar 5];30(3):207–21. Available from: https://www.dl.begellhouse.com/journals/6dbf508d3b17c437,42112eb27fee5114,06731e962d6b02ed.html.
    DOI  |   Google Scholar
  25. Loic L, Marchand L, Seifried A, Lum-Jones A, Donlon T, Wilkens LR. Association of the Cyclin D1 A870G Polymorphisms With Advanced Colorectal Cancer. [cited 2022 Oct 5]; Available from: https://jamanetwork.com/.
     Google Scholar
  26. Xi Y, Xu P. Global colorectal cancer burden in 2020 and projections to 2040. Transl Oncol. 2021 Oct 1;14(10).
    DOI  |   Google Scholar
  27. Jun S-Y, Kim J, Yoon N, Maeng L-S, Byun JH. Prognostic Potential of Cyclin D1 Expression in Colorectal Cancer. J Clin Med [Internet]. 2023 Jan 10 [cited 2023 Jan 31];12(2). Available from: http://www.ncbi.nlm.nih.gov/pubmed/36675501.
    DOI  |   Google Scholar
  28. Saul D, Kosinsky RL. Single-Cell Transcriptomics reveals the expression of aging-and senescence-associated genes in distinct cancer cell populations. 2021; Available from: https://doi.org/10.3390/cells10113126.
    DOI  |   Google Scholar
  29. Gunasekaran V, Ekawati NP, Sumadi IWJ. Karakteristik klinikopatologi karsinoma kolorektal di RSUP Sanglah, Bali, Indonesia tahun 2013–2017. Intisari Sains Medis [Internet]. 2019 Dec 1;10(3). Available from: https://isainsmedis.id/index.php/ism/article/view/458. Indonesian.
    DOI  |   Google Scholar
  30. Perna C, Navarro A, Ruz-Caracuel I, Caniego-Casas T, Cristóbal E, Leskelä S, et al. Molecular heterogeneity of high-grade colorectal adenocarcinoma. Cancers (Basel). 2021;13(2).
    DOI  |   Google Scholar
  31. Indrayani Maker LPINS, Sriwidyani NP. Hubungan antara status tumor budding dengan berbagai parameter klinikopatologi pada adenokarsinoma kolorektal di RSUP Sanglah, Bali, Indonesia. Intisari Sains Medis [Internet]. 2021 Dec 7 [cited 2022 Oct 26];12(3):842–7. Available from: https://isainsmedis.id/index.php/ism/article/view/1123. Indonesian.
    DOI  |   Google Scholar
  32. Joshi AD, Chan AT. Racial differences in epigenetic aging of the colon: implications for colorectal cancer. Available from: https://academic.oup.com/jnci/article/113/12/1618/6055552.
    DOI  |   Google Scholar
  33. Baran B, Mert Ozupek N, Yerli Tetik N, Acar E, Bekcioglu O, Baskin Y. Difference between left-sided and right-sided colorectal cancer: a focused review of literature. Gastroenterol Res [Internet]. 2018;11(4):264–73. Available from: http://www.gastrores.org/index.php/Gastrores/article/view/1062.
    DOI  |   Google Scholar
  34. Hong Y, Kong WE, Seow-Choen F, Fook-Chong S, Peh YC. GG genotype of cyclin D1 G870A polymorphisms is associated with increased risk and advanced colorectal cancer in patients in Singapore. Eur J Cancer [Internet]. 2005 [cited 2023 Mar 4];41(7):1037–44. Available from: https://pubmed.ncbi.nlm.nih.gov/15862753/.
    DOI  |   Google Scholar
  35. Ohnishi Y, Watanabe M, Wato M, Tanaka A, Kakudo K, Nozaki M. Cyclin D1 expression is correlated with cell differentiation and cell proliferation in oral squamous cell carcinomas. Oncol Lett [Internet]. 2014 Apr [cited 2023 Mar 12];7(4):1123. Available from: /pmc/articles/PMC3961451/.
    DOI  |   Google Scholar
  36. Yang Y, Wang F, Shi C, Zou Y, Qin H, Ma Y. Cyclin D1 G870A polymorphisms contributes to colorectal cancer susceptibility: evidence from a systematic review of 22 case-control studies. PLoS One [Internet]. 2012 May 11 [cited 2023 Feb 5];7(5): e36813. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036813.
    DOI  |   Google Scholar
  37. Thakur N, Kumari S, Mehrotra R. Association between Cyclin D1 G870A (rs9344) polymorphisms and cancer risk in Indian population: Meta-analysis and trial sequential analysis. Biosci Rep. 2018 Nov 30;38(6).
    DOI  |   Google Scholar
  38. Kastrinos F, Samadder NJ, Burt RW. Use of family history and genetic testing to determine the risk of colorectal cancer. Gastroenterology. 2020 Jan 1;158(2):389–403.
    DOI  |   Google Scholar
  39. Baziad A. Polimorfisme sebagai suatu uji genetik: Sebuah tinjauan kritis. Indones J Obstet Gynecol [Internet]. 2007 [cited 2023 Feb 13]; Available from: https://inajog.com/index.php/journal/article/view/123. Indonesian.
     Google Scholar
  40. Garibyan L, Avashia N. Polymerase chain reaction. J Invest Dermatol. 2013;133(3):1–4.
    DOI  |   Google Scholar
  41. Yang, S.-M. 2016. Correlation analysis of G870A CCND1 gene polymorphism with digestive system tumors. Journal of Southern Medical University, 36(11). Availabe: https://pubmed.ncbi.nlm.nih.gov/27881356/.
     Google Scholar