The Ethanol Extract of Tangse Liberica Coffee (Coffea liberica) Inhibited the Reduction of Leydig Cell Number and Testosterone Levels in Male Wistar Rats (Rattus norvegicus) Exposed to Ultraviolet B Light
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Background: Aging is a complex mechanism related to various factors including free radicals. Free radicals that occur due to exposure to ultraviolet light can cause oxidative stress and damage the spermatogenesis process, including Leydig cells and testosterone. Therefore, antioxidants play a very important role in inhibiting these reactions, which are abundant in coffee. This study aimed to analyze the effect of the ethanol extract of Tangse liberica coffee (Coffea liberica) in inhibiting the decrease in Leydig cell number and testosterone levels in male Wistar (Rattus norvegicus) rats exposed to Ultraviolet B light.
Methods: This study includes a post-test-only control group type of laboratory experimental study. The research group was divided into three groups, namely positive control (without giving coffee), treatment 1 (giving coffee extract 216 mg/kgBW), and treatment 2 (giving coffee extract 432 mg/kg BW). A total of 30 rats were used in this study and exposed to UV-B 0.225 MW/cm² 8 hours per day for 28 days. Testosterone assessment was carried out by examining blood specimens and Leydig cell counts were carried out histologically with Hematoxylin-Eosin staining. One-way ANOVA analysis and Kruskal Wallis test followed by post hoc test were performed in this study with a significance level of 95%.
Results: The result showed a significant difference in the number of Leydig cells between the study groups (p = 0.016) with the highest mean in group 1, 48.27 9.12 cells/LFoV. The same thing also happened to the difference in testosterone levels between groups (p < 0.001) with the most dominant mean in treatment group 2 being 76.42 nmol/L.
Conclusion: The conclusion of this research is the administration of the ethanol extract of Tangse liberica coffee (Coffea liberica) can inhibit the decrease in Leydig cells number and testosterone levels in male Wistar (Rattus norvegicus) rats exposed to Ultraviolet B light.
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References
-
Lemoine M. Defining aging. Biol Philos. Springer; 2020;35:1–30.
DOI | Google Scholar
1
-
Bulterijs S, Hull RS, Björk VCE, Roy AG. It is time to classify biological aging as a disease. Front Genet., 2015;205.
DOI | Google Scholar
2
-
Mercado-Sáenz S, Ruiz-Gómez MJ, Morales-Moreno F, Martínez-Morillo M. Cellular aging: theories and technological influence. Brazilian Arch Biol Technol., 2010;53:1319–32.
DOI | Google Scholar
3
-
De Jager TL, Cockrell AE, Du Plessis SS. Ultraviolet light induced generation of reactive oxygen species. Ultrav Light Hum Heal Dis Environ, 2017;15–23.
DOI | Google Scholar
4
-
Amaro-Ortiz A, Yan B, D’Orazio JA. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules, 2014;19:6202–19.
DOI | Google Scholar
5
-
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxid Med Cell Longev, 2017;2017.
DOI | Google Scholar
6
-
Pereira SC, Oliveira PF, Oliveira SR, Pereira M de L, Alves MG. Impact of environmental and lifestyle use of chromium on male fertility: focus on antioxidant activity and oxidative stress. Antioxidants, 2021;10:1365.
DOI | Google Scholar
7
-
Ismalia KR, Pangkahila W, Sriwidyani NP. Oral administration of Bali Robusta coffee (Coffea canephora) extract prevented the reduction of Leydig cells and testosterone levels in male Wistar rats (Rattus norvegicus) with excessive physical training. Neurol Spinale Med Chir., 2021;4:37–41.
DOI | Google Scholar
8
-
Omu AE. Sperm parameters: paradigmatic index of good health and longevity. Med Princ Pract., 2013;22:30–42.
DOI | Google Scholar
9
-
Yashin A, Yashin Y, Wang JY, Nemzer B. Antioxidant and antiradical activity of coffee. Antioxidants, 2013;2:230–45.
DOI | Google Scholar
10
-
Saw AK-C, Yam W-S, Wong K-C, Lai C-S. A comparative study of the volatile constituents of southeast Asian Coffea arabica, Coffea liberica and Coffea robusta green beans and their antioxidant activities. J Essent Oil Bear Plants, 2015;18:64–73.
DOI | Google Scholar
11
-
Badan Pertanahan Nasional Provinsi Aceh. Kopi Tangse, potensi alam yang terabaikan dari pedalaman Pidie. Banda Aceh, 2021.
Google Scholar
12
-
Procházková D, Boušová I, Wilhelmová N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia, 2011;82:513–23.
DOI | Google Scholar
13
-
Insanu M, Fidrianny I, Imtinan NHH, Kusmardiyani S. Liberica Coffee (Coffea liberica L.) from Three Different Regions: In Vitro Antioxidant Activities. AMG Transcend Association, 2021;
Google Scholar
14
-
Heywood, L.H. Testosterone Levels in the Male Laboratory Rat: Variation under Experimental Conditions. Int J Androl, 2010;3:519–529. https://doi.org/10.1111/j.1365-2605.1980.tb00140.x.
DOI | Google Scholar
15
-
Khalaji N, Namyari M, Rasmi Y, Pourjabali M, Chodari L. Protective effect of curcumin on fertility of rats after exposure to compact fluorescent lamps: An experimental study. Int J Reprod Biomed., 2018;16:447.
DOI | Google Scholar
16
-
Hartono KM, Ariani MD, Wibowo DA. Pengaruh Pemberian Kopi Terhadap Motilitas Spermatozoa Tikus Wistar Yang Dipapar Sinar Ultraviolet. Diponegoro University; 2016.
Google Scholar
17
-
de Oliveira Silva E, Batista R. Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: a review on their structures, occurrence, and potential health benefits. Compr Rev Food Sci Food Saf. Wiley Online Library, 2017;16:580–616.
DOI | Google Scholar
18
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